Plasmid

Part:BBa_J100267:Design

Designed by: Owen Koucky   Group: Campbell M Lab   (2016-06-17)


pJC174c-R5 (Carlson et al. 2014)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3516
    Illegal AgeI site found at 1424
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3224
    Illegal SapI site found at 2322
    Illegal SapI.rc site found at 767


Design Notes

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014


Source

"Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., 2014

References